Rat MFGE8(Milk Fat Globule EGF Factor 8) ELISA Kit

Mfr.No.

ELK7230

Alternative Names

HMFG; MFGM; SED1; BA46; EDIL1; OAcGD3S; SPAG10; hP47; Medin; Lactadherin; Sperm Associated Antigen 10; Sperm Surface Protein hP47; Milk fat globule-EGF factor 8

Reactivity

Rat

Detection Range

15.63-1000 pg/mL

Sample Type

Cell lysates, Serum, Cell culture supernatants, Plasma, Tissue homogenates, Other biological fluids

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant MFGE8 and the recovery rates were calculated by comparing the measured value to the expected amount of MFGE8 in samples.

Matrix	Recovery range	Average
serum(n=5)	92-106%	99%
EDTA plasma(n=5)	90-105%	97%
Heparin plasma(n=5)	88-103%	95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MFGE8 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-107%	84-98%	95-103%	86-98%
EDTA plasma(n=5)	85-96%	87-101%	89-102%	85-98%
Heparin plasma(n=5)	85-97%	87-96%	86-98%	91-103%

Typical Data

Concentration (pg/mL)	OD	Corrected OD
1000.00	2.164	2.069
500.00	1.694	1.599
250.00	1.128	1.033
125.00	0.728	0.633
62.50	0.547	0.452
31.25	0.305	0.210
15.63	0.198	0.103
0.00	0.095	0.000

Sensitivity

5.9 pg/mL

Limit Of Detection

1000 pg/mL

UniProt ID	P70490
Antigen	MFGE8
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MFGE8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MFGE8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MFGE8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MFGE8 in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Tumor immunity;
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15204693	ELK7230	48T	$320		Add to Cart
BHE15204693	ELK7230	96T	$458		Add to Cart

Rat MFGE8(Milk Fat Globule EGF Factor 8) ELISA Kit

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