Rat Membrane primary amine oxidase(AOC3) ELISA kit

Rat Membrane primary amine oxidase(AOC3) ELISA kit

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Mfr.No.

CSB-EL001855RA

Sensitivity

1.95 ng/mL

Detection Range

7.8 ng/mL-500 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

amine oxidase, copper containing 3 (vascular adhesion protein 1), HPAO, SSAO, VAP-1, VAP1, amine oxidase, copper containing 3, copper amine oxidase, semicarbazide-sensitive amine oxidase, vascular adhesion protein 1

Recovery

The recovery of mouse trypsin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	95	89-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse trypsin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	89-95
1:2	Average %	103
	Range %	99-107
1:4	Average %	92
	Range %	87-96
1:8	Average %	98
	Range %	94-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.219	2.112	2.166	2.010
50	1.624	1.576	1.600	1.444
25	1.001	0.991	0.996	0.840
12.5	0.679	0.666	0.673	0.517
6.25	0.377	0.356	0.367	0.211
3.12	0.304	0.313	0.309	0.153
1.56	0.260	0.273	0.267	0.111
0	0.155	0.157	0.156	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	O08590

Applications	ELISA
Research Area	AOC3

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508336	CSB-EL001855RA	96T	$595	Add to Cart
BHE10508336	CSB-EL001855RA	5x96T	$2856	Add to Cart
BHE10508336	CSB-EL001855RA	10x96T	$5652.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.