Rat MCP-1 ELISA Kit PicoKine®

Mfr.No.

EK0902

Sensitivity

<10 pg/ml

Detection Range

15.6 pg/ml - 1,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.002	0.024	0.058	0.137	0.266	0.491	1.137	1.885

Description

Rat MCP-1 ELISA Kit PicoKine® (96 Tests). Quantitate Rat CCL2 in cell culture supeatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

C-C motif chemokine 2;Immediate-early serum-responsive protein JE;Monocyte chemoattractant protein 1;Monocyte chemotactic protein 1;MCP-1;Small-inducible cytokine A2;CCL2;Je, Mcp1, Scya2;

Molecular Weight

16460 MW

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q24-N148

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural and recombinant rat MCP-1

Subcellular Localization

Secreted.

Sample Type

Cell culture supeatants, Serum

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	41	257	473	40	251	504
Standard deviation	2.29	21.58	27.43	2.56	21.58	29.23
CV (%)	7.2%	7.2%	5.8%	6.4%	8.6%	5.8%

Protein Function

Chemotactic factor that attracts monocytes, but not neutrophils.

Background

Monocyte chemoattractant protein-1 (MCP-1), a member of the chemokine (chemotactic cytokine) family, is a potent monocyte agonist that is upregulated by oxidized lipids.1 MCP-1 is also known as CCL2, SCYA2, MCAF. MCAF is a member of family of factors involved in immune and inflammatory responses. The amino acid sequence deduced from the nucleotide sequence reveals the primary structure of the MCAF precursor to be composed of a putative signal peptide sequence of 23 amino acid residues and a mature MCAF sequence of 76 amino acid residues.2 MCP-1 plays a unique and crucial role in the initiation of atherosclerosis and may provide a new therapeutic target in this disorder.3

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	41	37	39	42	39	1.92	4.9%
Sample 2	257	231	216	228	233	14.94	6.4%
Sample 3	473	462	446	461	460	9.6	2%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene ID	24770
Gene Name	CCL2
UniProt ID	P14844
Protein Name	C-C motif chemokine 2

Applications	ELISA
Research Area	Angiogenesis,Atherosclerosis,Cancer,Cancer Metabolism,Cardiovascular,Chemokines,Heart Disease,Immunology,Innate Immunity,Invasion/Microenvironment,Metabolism,Metabolism Processes,Pathways and Processes,Response To Hypoxia,Vascular Inflammation

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles. (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000683	EK0902	96 wells/kit	$499		Add to Cart

Rat MCP-1 ELISA Kit PicoKine®

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