Rat Matrix Metalloproteinase 9

Mfr.No.

E0321Ra

Sensitivity

0.01ng/ml

Detection Range

0.05-10ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Matrix Metalloproteinase 9 (also known as MMP-9) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

92 kDa gelatinase;92 kDa type IV collagenase;Gelatinase B;GELB;Matrix Metalloproteinase-9;MMP 9;MMP9;MMP-9

Molecular Weight

78611

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0321Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	3.5	0.14	4
Intra-Assay	2	18	1.3	0.07	5.9
Intra-Assay	3	18	2.7	0.17	6.1

Background

Could play a role in bone osteoclastic resorption. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments (By similarity).

Protocol

These standard curves of E0321Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
6.4ng/ml	2.077	2.089	2.06
	2.101
3.2ng/ml	1.243	1.279	1.251
	1.316
1.6ng/ml	0.7	0.774	0.745
	0.848
0.8ng/ml	0.519	0.478	0.449
	0.438
0.4ng/ml	0.21	0.201	0.172
	0.192
0ng/ml	0.03	0.029	0
	0.028

Gene ID	81687
UniProt ID	P50282

Research Area	Cardiovascular; Cancer; Cell Biology

Storage	2-8°C

Reference	Oral Doxycycline on the Level of Matrix Metalloproteinase 9 in Rat Models Experiencing Traumatic Brain Injury. Journal: IIUM Medical Journal Malaysia, 20(1):11-16

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111620	E0321Ra	48T	$320		Add to Cart
BHE12111620	E0321Ra	96T	$458		Add to Cart

Rat Matrix Metalloproteinase 9 - MMP-9 ELISA Kit

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