Rat matrix metalloproteinase 7,MMP-7 ELISA kit

Rat matrix metalloproteinase 7,MMP-7 ELISA kit

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Mfr.No.

CSB-E07408r

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

matrix metallopeptidase 7 (matrilysin, uterine), MMP-7, MPSL1, PUMP-1, matrin, matrix metalloproteinase 7, matrix metalloproteinase 7 (matrilysin, uterine), uterine matrilysin

Recovery

The recovery of human ROBO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-98
EDTA plasma (n=4)	92	87-96

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ROBO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	84-98
1:2	Average %	96
	Range %	90-100
1:4	Average %	89
	Range %	85-94
1:8	Average %	87
	Range %	81-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.120	1.978	2.049	1.929
5	1.575	1.546	1.561	1.441
2.5	0.958	0.904	0.931	0.811
1.25	0.541	0.539	0.540	0.420
0.625	0.352	0.324	0.338	0.218
0.312	0.232	0.219	0.226	0.106
0.156	0.178	0.169	0.174	0.054
0	0.122	0.117	0.120	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P50280

Applications	ELISA
Research Area	MMP7

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508331	CSB-E07408r	96T	$615	Add to Cart
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BHE10508331	CSB-E07408r	10x96T	$5842.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.