Rat Matrix Metalloproteinase 3

Mfr.No.

E0316Ra

Sensitivity

0.07ng/ml

Detection Range

0.2-60ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Matrix Metalloproteinase 3 (also known as MMP-3) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Matrix Metalloproteinase-3;MMP-3;PTR1 protein;SL-1;Stromelysin-1;Transin-1

Molecular Weight

53428

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0316Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	6.44	0.43	6.65
Intra-Assay	2	18	12.79	0.67	5.22
Intra-Assay	3	18	3.92	0.25	6.3

Background

Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.

Protocol

These standard curves of E0316Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
32ng/ml	2.244	2.301	2.264
	2.357
16ng/ml	1.861	1.849	1.812
	1.837
8ng/ml	1.268	1.195	1.158
	1.122
4ng/ml	0.758	0.731	0.695
	0.705
2ng/ml	0.367	0.383	0.346
	0.398
0ng/ml	0.041	0.037	0
	0.032

Gene ID	171045
UniProt ID	P03957

Research Area	Cardiovascular; Cancer; Signal Transduction; Cell Biology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111615	E0316Ra	48T	$320		Add to Cart
BHE12111615	E0316Ra	96T	$458		Add to Cart

Rat Matrix Metalloproteinase 3 - MMP-3 ELISA Kit

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