Rat matrix metalloproteinase 2/Gelatinase A,MMP-2 ELISA kit

Rat matrix metalloproteinase 2/Gelatinase A,MMP-2 ELISA kit

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Mfr.No.

CSB-E07411r

Sensitivity

0.156 ng/mL

Detection Range

0.625 ng/mL-40 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase), CLG4, CLG4A, MMP-II, MONA, TBE-1, collagenase type IV-A, matrix metalloproteinase 2, matrix metalloproteinase-II, neutrophil gelatinase

Recovery

The recovery of human RNASE7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	95-105
EDTA plasma (n=4)	104	100-109

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human RNASE7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	100
	Range %	95-105
1:2	Average %	98
	Range %	93-103
1:4	Average %	89
	Range %	80-95
1:8	Average %	88
	Range %	82-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
100	2.410	2.456	2.433	2.256
50	1.618	1.629	1.624	1.447
25	1.076	1.098	1.087	0.910
12.5	0.726	0.703	0.715	0.538
6.25	0.490	0.483	0.487	0.310
3.12	0.384	0.354	0.369	0.192
1.56	0.269	0.282	0.276	0.099
0	0.178	0.176	0.177	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P33436

Applications	ELISA
Research Area	MMP2

Storage	Store at 2-8°C. Please refer to protocol.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.