Rat Mast Cell Tryptase,MCT ELISA Kit

Rat Mast Cell Tryptase,MCT ELISA Kit

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Mfr.No.

CSB-E13627r

Sensitivity

0.156 ng/mL

Reactivity

Rat

Sample Type

Serum, Urine, Plasma

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

tryptase alpha/beta 1, TPS1, TPS2, TPSB1, mast cell alpha II tryptase, mast cell beta I tryptase, tryptase alpha II, tryptase beta 1, tryptase beta I, tryptase-I, tryptase-III

Recovery

The recovery of human CD226 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	100	95-105
EDTA plasma (n=4)	97	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CD226 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	85-91
1:2	Average %	90
	Range %	87-93
1:4	Average %	93
	Range %	90-96
1:8	Average %	88
	Range %	84-92

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
60	2.801	2.867	2.834	2.732
30	1.866	1.832	1.849	1.747
15	1.216	1.264	1.240	1.138
7.5	0.799	0.754	0.777	0.675
3.75	0.363	0.367	0.365	0.263
1.8	0.256	0.251	0.254	0.152
0.9	0.170	0.173	0.172	0.070
0	0.101	0.103	0.102

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P27435
Antigen	tryptase alpha/beta 1

Applications	ELISA
Research Area	TPSAB1

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508321	CSB-E13627r	96T	$790	Add to Cart
BHE10508321	CSB-E13627r	5x96T	$3792	Add to Cart
BHE10508321	CSB-E13627r	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.