Rat Mast Cell Protease 1 - MCPT1 ELISA Kit

Mfr.No.

E2349Ra

Sensitivity

0.043ng/mL

Detection Range

0.1-40ng/mL

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Chymase;Chymotrypsin-like protease;CLIP protein;Mast cell protease 1;Mast cell protease I;Mcpt1;rMCP-1;rMCP-I

Molecular Weight

28617

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E2349Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	5.84	0.32	5.42
Intra-Assay	2	18	12.11	0.34	2.79
Intra-Assay	3	18	4.65	0.29	6.32

Background

Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. May participate in generating perivascular beta-protein which ultimately aggregates into amyloid-beta deposits.

Protocol

These standard curves of E2349Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
24ng/mL	2.267	2.285	2.232
	2.302
12ng/mL	1.519	1.533	1.481
	1.548
6ng/mL	0.759	0.827	0.775
	0.896
3ng/mL	0.464	0.434	0.382
	0.404
1.5ng/mL	0.229	0.268	0.216
	0.308
0ng/mL	0.047	0.052	0
	0.057

Gene ID	100360872
UniProt ID	P09650

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12115453	E2349Ra	48T	$320		Add to Cart
BHE12115453	E2349Ra	96T	$458		Add to Cart

Rat Mast Cell Protease 1 - MCPT1 ELISA Kit

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