Rat MAP1LC3B(Microtubule-associated proteins 1A/1B light chain 3B) ELISA Kit

Mfr.No.

ELK8900

Alternative Names

LC3B; MAP1LC3B; MAP1ALC3; Microtubule-associated proteins 1A/1B light chain 3B; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP

Reactivity

Rat

Detection Range

0.16-10 ng/mL

Sample Type

Tissue homogenates, Cell lysates, Other biological fluids

Assay Type

Sandwich

Assay Duration

3.5h

Recovery

Matrices listed below were spiked with certain level of recombinant MAP1LC3B and the recovery rates were calculated by comparing the measured value to the expected amount of MAP1LC3B in samples.

Matrix	Recovery range	Average
serum(n=5)	94-105%	98%
EDTA plasma(n=5)	82-94%	88%
Heparin plasma(n=5)	83-99%	95%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of MAP1LC3B and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	85-96%	95-104%	93-102%	79-94%
EDTA plasma(n=5)	85-100%	80-97%	81-92%	87-103%
Heparin plasma(n=5)	87-99%	85-96%	87-101%	89-102%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
10.00	2.126	2.017
5.00	1.625	1.516
2.50	1.144	1.035
1.25	0.892	0.783
0.63	0.563	0.454
0.32	0.315	0.206
0.16	0.176	0.067
0.00	0.109	0.000

Sensitivity

0.067 ng/mL

Limit Of Detection

10 ng/mL

UniProt ID	Q62625
Antigen	MAP1LC3B
Usage Notes	The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat MAP1LC3B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat MAP1LC3B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat MAP1LC3B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat MAP1LC3B in the samples is then determined by comparing the OD of the samples to the standard curve.

Applications	ELISA
Research Area	Metabolic pathway
Protocol	Datasheet
Precision	Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

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SKU	Supplier No.	Size	Your price	Quantity
BHE15204359	ELK8900	48T	$265		Add to Cart
BHE15204359	ELK8900	96T	$376		Add to Cart

Rat MAP1LC3B(Microtubule-associated proteins 1A/1B light chain 3B) ELISA Kit

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