Rat Malate Dehydrogenase - Cytoplasmic

Mfr.No.

E2140Ra

Alternative Names

Cytosolic malate dehydrogenase;Malate dehydrogenase, cytoplasmic;MDH 1;MDH1

Reactivity

Rat

Detection Range

3-900mU/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

36483

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

1.355mU/mL

Background

Catalyzes the reduction of aromatic alpha-keto acids in the presence of NADH. Plays essential roles in the malate-aspartate shuttle and the tricarboxylic acid cycle, important in mitochondrial NADH supply for oxidative phosphorylation.

Gene ID	24551
UniProt ID	O88989
Antigen	MDH1

Research Area

Others

Protocol

These standard curves of E2140Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
480mU/mL	1.99	1.978	1.923
	1.967
240mU/mL	1.057	1.079	1.024
	1.102
120mU/mL	0.425	0.424	0.369
	0.423
60mU/mL	0.204	0.219	0.164
	0.234
30mU/mL	0.088	0.092	0.037
	0.097
0mU/mL	0.046	0.055	0
	0.064

Precision

We measured random samples of E2140Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	344.7	11.88	3.4
Intra-Assay	2	18	174	6.16	3.5
Intra-Assay	3	18	239.2	10.6	4.4

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12113272	E2140Ra	48T	$320		Add to Cart
BHE12113272	E2140Ra	96T	$458		Add to Cart

Rat Malate Dehydrogenase - Cytoplasmic - MDH1 ELISA Kit

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