Rat Macrophage Migration Inhibitory Factor,MIF ELISA Kit

Mfr.No.

CSB-E07293r

Sensitivity

15.6 pg/mL

Detection Range

62.5 pg/mL-4000 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Alternative Names

macrophage migration inhibitory factor (glycosylation-inhibiting factor), GIF, GLIF, MMIF, macrophage migration inhibitory factor, phenylpyruvate tautomerase

Recovery

The recovery of canine RLN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	101-107
EDTA plasma (n=4)	99	94-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of canine RLN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	87
	Range %	84-90
1:2	Average %	95
	Range %	91-99
1:4	Average %	88
	Range %	84-93
1:8	Average %	97
	Range %	93-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.366	2.504	2.435	2.269
100	1.644	1.602	1.623	1.457
50	0.901	0.964	0.933	0.767
25	0.487	0.468	0.478	0.312
12.5	0.319	0.327	0.323	0.157
6.25	0.261	0.268	0.265	0.099
3.12	0.206	0.196	0.201	0.035
0	0.161	0.171	0.166	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Protocol

UniProt ID	P30904

Applications	ELISA
Research Area	MIF

Storage	Store at 2-8°C. Please refer to protocol.

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Rat Macrophage Migration Inhibitory Factor,MIF ELISA Kit

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