Rat macrophage inflammatory protein 3alpha,MIP-3alpha ELISA Kit

Rat macrophage inflammatory protein 3alpha,MIP-3alpha ELISA Kit

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Mfr.No.

CSB-E04669r

Sensitivity

0.039 ng/ml

Reactivity

Rat

Sample Type

Serum, Tissue homogenates, Plasma

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

chemokine (C-C motif) ligand 20, CKb4, LARC, MIP-3a, MIP3A, SCYA20, ST38, exodus-1, macrophage inflammatory protein 3 alpha, small inducible cytokine subfamily A (Cys-Cys), member 20, MIP-3 alpha

Recovery

The recovery of rabbit cTn-? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	83-94
EDTA plasma (n=4)	89	85-97

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rabbit cTn-? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	81-92
1:2	Average %	96
	Range %	92-99
1:4	Average %	94
	Range %	89-98
1:8	Average %	92
	Range %	88-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
3000	2.754	2.559	2.657	2.564
1500	2.115	2.020	2.068	1.975
750	1.248	1.227	1.238	1.145
375	0.658	0.677	0.668	0.575
187.5	0.403	0.412	0.408	0.315
94	0.264	0.278	0.271	0.178
47	0.151	0.147	0.149	0.056
0	0.093	0.092	0.093

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P97884
Antigen	chemokine (C-C motif) ligand 20

Applications	ELISA
Research Area	CCL20

Storage	Store at 2-8°C. Please refer to protocol.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.