Rat macrophage inflammatory protein 2,MIP-2 ELISA kit

Rat macrophage inflammatory protein 2,MIP-2 ELISA kit

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Mfr.No.

CSB-E07419r

Sensitivity

19.5 pg/mL

Detection Range

78 pg/mL-5000 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Sample Volume

50-100ul

Alternative Names

chemokine (C-X-C motif) ligand 2, CINC-2a, GRO2, GROb, MGSA-b, MIP-2a, MIP2, MIP2A, SCYB2, GRO2 oncogene, MGSA beta, melanoma growth stimulatory activity beta

Recovery

The recovery of mouse ECP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-97
EDTA plasma (n=4)	95	89-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse ECP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-98
1:2	Average %	97
	Range %	92-106
1:4	Average %	93
	Range %	88-99
1:8	Average %	89
	Range %	83-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.546	2.652	2.599	2.435
5	2.074	2.076	2.075	1.911
2.5	1.413	1.504	1.459	1.295
1.25	0.676	0.735	0.706	0.542
0.625	0.486	0.524	0.505	0.341
0.312	0.384	0.359	0.372	0.208
0.156	0.251	0.259	0.255	0.091
0	0.163	0.165	0.164	?

Species

Rattus norvegicus

Sample Type

Serum, Tissue homogenates, Plasma

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P30348
Tissue Specificity	At least expressed in the lung and trachea.

Applications	ELISA
Research Area	CXCL2

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508306	CSB-E07419r	96T	$495	Add to Cart
BHE10508306	CSB-E07419r	5x96T	$2376	Add to Cart
BHE10508306	CSB-E07419r	10x96T	$4702.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.