Rat Macrophage Inflammatory Protein 1alpha

Mfr.No.

E0369Ra

Sensitivity

3.11ng/L

Detection Range

7-1500ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

C-C motif chemokine 3;CCL 3;CCL3;Macrophage inflammatory protein 1-alpha;MIP-1-alpha;Small-inducible cytokine A3

Molecular Weight

10335

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0369Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	550.1	15.08	2.7
Intra-Assay	2	18	246.2	10.91	4.4
Intra-Assay	3	18	109.4	6.58	6

Background

Monokine with inflammatory and chemokinetic properties. Has chemotactic activity for monocytes, neutrophils, eosinophils, basophils, and lymphocytes. Required for lung TNF-alpha production, neutrophil recruitment and subsequent lung injury and may function as an autocrine mediator for the macrophage production of TNF-alpha which in turn up-regulates vascular adhesion molecules required for neutrophil influx. This protein binds heparin.

Protocol

These standard curves of E0369Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800ng/L	2.118	2.179	2.106
	2.24
400ng/L	1.347	1.329	1.257
	1.311
200ng/L	0.838	0.813	0.74
	0.787
100ng/L	0.4	0.417	0.345
	0.435
50ng/L	0.254	0.287	0.215
	0.321
0ng/L	0.078	0.073	0
	0.067

Gene ID	25542
UniProt ID	P50229

Research Area	Immunology

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111665	E0369Ra	48T	$320		Add to Cart
BHE12111665	E0369Ra	96T	$458		Add to Cart

Rat Macrophage Inflammatory Protein 1alpha - MIP1A ELISA Kit

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