Rat Macrophage Colony-Stimulating Factor,M-CSF ELISA kit

Rat Macrophage Colony-Stimulating Factor,M-CSF ELISA kit

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Mfr.No.

CSB-E07424r

Alternative Names

colony stimulating factor 1 (macrophage), MCSF, MGC31930, OTTHUMP00000013363, OTTHUMP00000013364, colony stimulating factor 1, macrophage colony stimulating factor

Reactivity

Rat

Detection Range

15.6 pg/mL-1000 pg/mL

Sample Type

Serum, Cell culture supernatants, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of pig S-100B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	83-102
EDTA plasma (n=4)	95	92-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig S-100B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-100
1:2	Average %	93
	Range %	85-101
1:4	Average %	86
	Range %	80-98
1:8	Average %	94
	Range %	92-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
5000	2.025	2.067	2.046	1.887
2500	1.574	1.524	1.549	1.390
1250	1.183	1.169	1.176	1.017
625	0.821	0.812	0.817	0.658
312	0.567	0.534	0.551	0.392
156	0.354	0.373	0.364	0.205
78	0.250	0.262	0.256	0.097
0	0.164	0.153	0.159	?

Species

Rattus norvegicus

Sensitivity

3.9 pg/mL

Limit Of Detection

450 nm

UniProt ID	Q8JZQ0
Antigen	colony stimulating factor 1 (macrophage)

Applications

ELISA

Research Area

CSF1

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508303	CSB-E07424r	96T	$615	Add to Cart
BHE10508303	CSB-E07424r	5x96T	$2952	Add to Cart
BHE10508303	CSB-E07424r	10x96T	$5842.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.