Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of LTF/LF and the recovery rates were calculated by comparing the measured value to the expected amount of LTF/LF in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-99
96
EDTA plasma(n=5)
85-100
93
heparin plasma(n=5)
87-101
94
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LTF/LF and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
LTF/LF ELISA Kit allows for the in vitro quantitative determination of LTF/LF concentrations in serum, plasma, tissue homogenates and other biological fluids.