Rat Low Density Lipoprotein Receptor

Mfr.No.

E1345Ra

Sensitivity

0.09ng/ml

Detection Range

0.2-60ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Low Density Lipoprotein Receptor (also known as LDLR) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

LDL receptor;LDLR;Low-density lipoprotein receptor

Molecular Weight

96622

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1345Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	17.2	0.73	4.3
Intra-Assay	2	18	8.4	0.34	4.1
Intra-Assay	3	18	6.3	0.26	4.2

Background

Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits.

Protocol

These standard curves of E1345Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
32ng/ml	2.085	2.114	2.076
	2.142
16ng/ml	1.458	1.503	1.466
	1.548
8ng/ml	0.586	0.617	0.579
	0.647
4ng/ml	0.404	0.365	0.327
	0.325
2ng/ml	0.165	0.142	0.104
	0.118
0ng/ml	0.043	0.037	0
	0.031

Gene ID	300438
UniProt ID	P35952

Research Area	Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112583	E1345Ra	48T	$320		Add to Cart
BHE12112583	E1345Ra	96T	$458		Add to Cart

Rat Low Density Lipoprotein Receptor - LDLR ELISA Kit

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