Matrices listed below were spiked with certain level of Lig1 and the recovery rates were calculated by comparing the measured value to the expected amount of Lig1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-103
94
EDTA plasma(n=5)
88-105
100
heparin plasma(n=5)
88-103
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lig1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Lig1 ELISA Kit allows for the in vitro quantitative determination of Lig1 concentrations in serum, plasma, tissue homogenates and other biological fluids.