Matrices listed below were spiked with certain level of LH and the recovery rates were calculated by comparing the measured value to the expected amount of LH in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
85-104
95
EDTA plasma(n=5)
85-103
93
heparin plasma(n=5)
91-103
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LH and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.