Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of Lgr5 and the recovery rates were calculated by comparing the measured value to the expected amount of Lgr5 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
90-103
97
EDTA plasma(n=5)
88-104
96
heparin plasma(n=5)
89-104
96
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lgr5 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
92-105%
85-96%
86-97%
EDTA plasma(n=5)
83-93%
82-98%
83-88%
heparin plasma(n=5)
87-100%
82-97%
83-93%
Sensitivity
< 9.375pg/ml
Notes
For Research Use Only
UniProt ID
D4AC13
Applications
ELISA
Applications Notes
LGR-5 ELISA Kit allows for the in vitro quantitative determination of Lgr5 concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat LGR-5 (leucine-rich repeat-containing g-protein coupled receptor 5) ELISA Kit