Rat leukotriene D4(LTD4) ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EQ027312RA

Sensitivity

1.95 pg/mL

Detection Range

7.8 pg/mL-500 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

leukotriene D4(LTD4)

Recovery

The recovery of rat CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	88-97
EDTA plasma (n=4)	96	91-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	89
	Range %	85-96
1:4	Average %	93
	Range %	89-97
1:8	Average %	99
	Range %	92-105

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	LTD4

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Enhanced lipoxygenase/LTD4 signaling accounts for the exaggerated hypertensive and nephrotoxic effects of cyclosporine plus indomethacin in rats Helmy MW.et al,Biomedicine & Pharmacotherapy,Mar 22

Have you used this product?

SKU	Supplier No.	Size	Your price
BHE10508279	CSB-EQ027312RA	96T	$790	Add to Cart
BHE10508279	CSB-EQ027312RA	5x96T	$3792	Add to Cart
BHE10508279	CSB-EQ027312RA	10x96T	$7505	Add to Cart