Rat Lep(Leptin) ELISA Kit

Mfr.No.

ER0115

Alternative Names

LEP ELISA Kit, Leptin ELISA Kit, LEPD ELISA Kit, OBS ELISA Kit, Obesity Homolog ELISA Kit

Reactivity

Rat

Detection Method

Double Antibody

Detection Range

0.156-10ng/ml

Sample Type

Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of Lep and the recovery rates were calculated by comparing the measured value to the expected amount of Lep in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	85-105	94
EDTA plasma(n=5)	89-101	94
heparin plasma(n=5)	85-103	94

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Lep and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	88-97%	89-100%	88-104%
EDTA plasma(n=5)	89-97%	84-100%	84-92%
heparin plasma(n=5)	90-97%	84-89%	80-100%

Sensitivity

< 0.094ng/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

LEP(Leptin)/LEPD/OBS/Obesity Homolog

Notes

For Research Use Only

UniProt ID	P50596
Antigen	Lep

Applications	ELISA
Applications Notes	Lep ELISA Kit allows for the in vitro quantitative determination of Lep concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10801004	ER0115	96T	$350		Add to Cart

Rat Lep (Leptin) ELISA Kit

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