Rat LDL(Low Density Lipoprotein) ELISA Kit

Mfr.No.

ELK7477

Sensitivity

2.71 ng/mL

Detection Range

6.25-400 ng/mL

Reactivity

Rat

Assay Duration

3.5h

Assay Type

Sandwich

Alternative Names

Low Density Lipoprotein

Recovery

Matrices listed below were spiked with certain level of recombinant LDL and the recovery rates were calculated by comparing the measured value to the expected amount of LDL in samples.

Matrix	Recovery range	Average
serum(n=5)	94-106%	100%
EDTA plasma(n=5)	80-97%	88%
Heparin plasma(n=5)	77-95%	86%

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LDL and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Matrix	1:2	1:4	1:8	1:16
serum(n=5)	95-106%	88-103%	81-98%	93-102%
EDTA plasma(n=5)	85-96%	86-98%	92-103%	87-93%
Heparin plasma(n=5)	81-94%	85-97%	93-102%	81-95%

Typical Data

Concentration (ng/mL)	OD	Corrected OD
400.00	1.946	1.853
200.00	1.674	1.581
100.00	1.279	1.186
50.00	0.828	0.735
25.00	0.555	0.462
12.50	0.388	0.295
6.25	0.216	0.123
0.00	0.093	0.000

Limit Of Detection

400 ng/mL

Precision

Intra-assay Precision (Precision within an assay)?CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)?CV%<10%
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.

Usage Notes

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat LDL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat LDL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat LDL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat LDL in the samples is then determined by comparing the OD of the samples to the standard curve.

Protocol

Datasheet

Applications	ELISA
Research Area	Metabolic pathway;Cardiovascular biology;Hepatology;Gastroenterology;Nutrition metabolism;

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SKU	Supplier No.	Size	Your price	Quantity
BHE15204614	ELK7477	48T	$320		Add to Cart
BHE15204614	ELK7477	96T	$458		Add to Cart

Rat LDL(Low Density Lipoprotein) ELISA Kit

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