Matrices listed below were spiked with certain level of LDL-C and the recovery rates were calculated by comparing the measured value to the expected amount of LDL-C in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
88-101
96
EDTA plasma(n=5)
85-101
92
heparin plasma(n=5)
87-103
93
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of LDL-C and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
88-105%
85-100%
92-105%
EDTA plasma(n=5)
87-97%
84-100%
86-95%
heparin plasma(n=5)
82-98%
81-100%
85-91%
Sensitivity
< 0.469ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
LDL-CELISA Kit allows for the in vitro quantitative determination of LDL-C concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat LDL-C(low density lipoprotein cholesterol)ELISA Kit