Rat Laminin ELISA Kit PicoKine®

Mfr.No.

EK0435

Sensitivity

<10 pg/ml

Detection Range

156 pg/ml - 10,000 pg/ml

Reactivity

Rat

Sample Volume

Concentration (pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.098	0.162	0.186	0.271	0.479	0.782	1.391	1.923

Description

Rat Laminin ELISA Kit PicoKine® (96 Tests). Quantitate Rat Laminin in cell culture supeatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Alternative Names

Laminin subunit beta-2;Laminin chain B3;Laminin-11 subunit beta;Laminin-14 subunit beta;Laminin-15 subunit beta;Laminin-3 subunit beta;Laminin-4 subunit beta;Laminin-7 subunit beta;Laminin-9 subunit beta;S-laminin subunit beta;S-LAM beta;Lamb2;

Molecular Weight

196474 MW

Immunogen

Target protein isolated from rat sarcoma basement membrane

Kit Components

Description Quantity
Pre-coated 96-well strip microplate 1
Standard 2 vials
Biotinylated antibody (100x) 100ul
Avidin-Biotin-Peroxidase Complex (100x) 100ul
Sample Diluent 30ml
Antibody Diluent 12ml
Avidin-Biotin-Peroxidase Diluent 12ml
Color Developing Reagent (TMB) 10ml
Stop Solution 10ml
Wash Buffer (25x) 20ml
Adhesive plate sealers 4

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Specificity

Natural rat Laminin

Subcellular Localization

Secreted, extracellular space, extracellular matrix, basement membrane.

Sample Type

Cell culture supeatants, Cell lysates, Serum, Plasma

Anticoagulant

heparin or EDTA

Precision

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (pg/ml)	256	1435	4770	279	1524	4389
Standard deviation	11.26	97.58	352.98	15.34	128.01	373.06
CV (%)	4.4%	6.8%	7.4%	5.5%	8.4%	8.5%

Protein Function

Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components.

Background

Laminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2. Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation. The nucleotide sequence of human laminin A chain has an open reading frame encoding 3075-amino acids. The human laminin A chain is at locus 18p11.3. The nucleotide sequence of the human laminin B1 reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide. The gene for the human laminin-B1 chain has been localized to chromosome 7, band q31. The B2 chain consists of six distinct domains, including two domains with alpha-helical, coiled-coil structures, two domains with cysteine-rich homologous repeats, and two globular domains. The amino acid sequences of the B2 and B1 chains demonstrate considerable homology. The human laminin B2 chain gene maps to the long arm of chromosome 1 in the band q31.

Reproducibility

Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
Sample 1	256	282	284	258	270	13.03	4.8%
Sample 2	1435	1315	1498	1568	1454	93.02	6.3%
Sample 3	4770	4867	4216	4900	4688	276.8	5.9%

*number of samples for each test n=16.

Notes

Material Required But Not Provided:
Microplate Reader capable of reading absorbance at 450nm.
Incubator.
Automated plate washer .
Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
Multichannel pipettes are recommended for large amount of samples.
Deionized or distilled water.
500ml graduated cylinders.
Test tubes for dilution.

Gene Name	LAMA1; LAMA2; LAMB1; LAMB2
UniProt ID	D4A409, F1M614, D3ZN05, F1LTF8, F1MAN8, D3ZQN7, P15800, F1LPI5, F1MAA7, F1LRH4, A0A0G2K023
Protein Name	Laminin subunit alpha-1; Laminin subunit alpha-2; Laminin subunit beta-1; Laminin subunit beta-2
Tissue Specificity	Found in the basement membranes (major component). S-laminin is concentrated in the synaptic cleft of the neuromuscular junction.
Sequence	Contains 13 laminin EGF-like domains.

Applications	ELISA
Research Area	Angiogenesis,Cardiovascular,Cell Adhesion Proteins,Cytoskeleton/ECM,ECM Proteins,Extracellular Matrix,Intermediate Filaments,Neuroscience,Signal Transduction

Storage	Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

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SKU	Supplier No.	Size	Your price	Quantity
BHE21000433	EK0435	96 wells/kit	$750		Add to Cart

Rat Laminin ELISA Kit PicoKine®

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