Rat Lactoylglutathione Lyase

Mfr.No.

E1640Ra

Sensitivity

0.29ng/ml

Detection Range

0.5-200ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Lactoylglutathione Lyase (also known as GLO1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

Aldoketomutase;GLO 1;GLO1;Glx I;Glyoxalase I;Ketone-aldehyde mutase;Lactoylglutathione lyase;Methylglyoxalase;S-D-lactoylglutathione methylglyoxal lyase;

Molecular Weight

20820

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1640Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	25.1	1.33	5.3
Intra-Assay	2	18	56.6	2.74	4.8
Intra-Assay	3	18	102.2	3.06	3

Background

Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione. Involved in the regulation of TNF-induced transcriptional activity of NF-kappa-B (By similarity).

Protocol

These standard curves of E1640Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
120ng/ml	1.9	1.939	1.891
	1.978
60ng/ml	0.975	0.954	0.906
	0.933
30ng/ml	0.522	0.538	0.49
	0.555
15ng/ml	0.254	0.234	0.186
	0.214
7.5ng/ml	0.106	0.101	0.053
	0.096
0ng/ml	0.061	0.048	0
	0.035

Gene ID	294320
UniProt ID	Q6P7Q4

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112846	E1640Ra	48T	$320		Add to Cart
BHE12112846	E1640Ra	96T	$458		Add to Cart

Rat Lactoylglutathione Lyase - GLO1 ELISA Kit

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