Rat L-Lactate Dehydrogenase,L-LDH ELISA Kit

Rat L-Lactate Dehydrogenase,L-LDH ELISA Kit

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Mfr.No.

CSB-E11324r

Alternative Names

lactate dehydrogenase A, GSD11, LDH1, LDHM, PIG19, LDH muscle subunit, lactate dehydrogenase M, proliferation-inducing gene 19, renal carcinoma antigen NY-REN-59

Reactivity

Rat

Detection Range

39 mU/mL-2500 mU/mL

Sample Type

Serum, Plasma, Tissue homogenates

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human CXCL3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	91-97
EDTA plasma (n=4)	97	93-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CXCL3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-93
1:2	Average %	100
	Range %	97-103
1:4	Average %	95
	Range %	92-97
1:8	Average %	99
	Range %	95-102

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
600	2.943	2.888	2.916	2.764
300	2.188	2.299	2.244	2.092
150	1.458	1.392	1.425	1.273
75	0.778	0.790	0.784	0.632
37.5	0.392	0.382	0.387	0.235
18.75	0.288	0.269	0.279	0.127
9.37	0.240	0.234	0.237	0.085
0	0.153	0.151	0.152	?

Species

Rattus norvegicus

Sensitivity

9.77 mU/mL

Limit Of Detection

450 nm

UniProt ID	P04642
Antigen	Lactate Dehydrogenase A

Applications

ELISA

Research Area

LDHA

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508287	CSB-E11324r	96T	$790	Add to Cart
BHE10508287	CSB-E11324r	5x96T	$3792	Add to Cart
BHE10508287	CSB-E11324r	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.