Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of Kynu and the recovery rates were calculated by comparing the measured value to the expected amount of Kynu in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
95-105
100
EDTA plasma(n=5)
95-103
98
heparin plasma(n=5)
95-105
99
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Kynu and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
92-98%
86-103%
98-105%
EDTA plasma(n=5)
89-98%
89-101%
90-100%
heparin plasma(n=5)
90-100%
91-93%
90-98%
Sensitivity
< 0.094ng/ml
Notes
For Research Use Only
UniProt ID
P70712
Applications
ELISA
Applications Notes
Kynu ELISA Kit allows for the in vitro quantitative determination of Kynu concentrations in serum, plasma, tissue homogenates and other biological fluids.