Rat Klotho ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E14958r

Sensitivity

31.25 pg/mL

Detection Range

125 pg/mL-8000 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

klotho

Recovery

The recovery of human I-TAC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	83-99
EDTA plasma (n=4)	97	92-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human I-TAC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	81-95
1:2	Average %	96
	Range %	90-105
1:4	Average %	97
	Range %	92-103
1:8	Average %	93
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q9Z2Y9

Applications	ELISA
Research Area	KL

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Klotho and S100A8/A9 as DiscriminativeMarkers between Pre-Renal and Intrinsic Acute Kidney Injury. Kim AJ.et al,PLoS One.,11(1):e0147255. Biological effects of dosing aerobic exercise and neuromuscular electrical stimulation in rats Dalise .et al,Sci Rep,Sep 7

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