Rat keyhole limpet hemocyanin (KLH) antibody (IgG)ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-EQ027435RA

Sensitivity

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Detection Range

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Reactivity

Rat

Assay Duration

1-5h

Assay Type

Indirect

Alternative Names

keyhole limpet hemocyanin (KLH) antibody (IgG)

Recovery

The recovery of mouse HAO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	92-103
EDTA plasma (n=4)	96	90-104

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse HAO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	83-107
1:2	Average %	95
	Range %	89-103
1:4	Average %	92
	Range %	85-105
1:8	Average %	91
	Range %	87-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	IgG

Storage	Store at 2-8°C. Please refer to protocol.

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BHE10508995	CSB-EQ027435RA	96T	$695	Add to Cart
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BHE10508995	CSB-EQ027435RA	10x96T	$6602.50	Add to Cart