Rat Keratinocyte Growth Factor,KGF ELISA Kit

Rat Keratinocyte Growth Factor,KGF ELISA Kit

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Mfr.No.

CSB-E12905r

Sensitivity

7.81 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

fibroblast growth factor 7 (keratinocyte growth factor), HBGF-7, KGF, fibroblast growth factor 7, heparin-binding growth factor 7, keratinocyte growth factor

Recovery

The recovery of human EN2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	83	80-87
EDTA plasma (n=4)	92	86-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human EN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-97
1:2	Average %	92
	Range %	86-96
1:4	Average %	97
	Range %	96-105
1:8	Average %	94
	Range %	89-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.466	2.365	2.416	2.271
10	1.759	1.658	1.709	1.564
5	1.028	1.007	1.018	0.873
2.5	0.615	0.604	0.610	0.465
1.25	0.391	0.402	0.397	0.252
0.625	0.258	0.268	0.263	0.118
0.312	0.221	0.224	0.223	0.078
0	0.144	0.145	0.145	?

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q02195

Applications	ELISA
Research Area	FGF7

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
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BHE10508248	CSB-E12905r	10x96T	$7505	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.