Rat keratinocyte autocrine factor/Amphiregulin,KAF/AR ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E07299r

Sensitivity

0.31 pg/mL

Detection Range

1.25 pg/mL-80 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

amphiregulin, AR, CRDGF, MGC13647, SDGF, colorectum cell-derived growth factor, schwannoma-derived growth factor

Recovery

The recovery of human TST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	89-98
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	95
	Range %	91-105
1:4	Average %	100
	Range %	92-110
1:8	Average %	92
	Range %	86-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P24338

Applications	ELISA
Research Area	AREG

Storage	Store at 2-8°C. Please refer to protocol.

Have you used this product?

SKU	Supplier No.	Size	Your price
BHE10508247	CSB-E07299r	96T	$595	Add to Cart
BHE10508247	CSB-E07299r	5x96T	$2856	Add to Cart
BHE10508247	CSB-E07299r	10x96T	$5652.50	Add to Cart