Rat Involucrin - INV ELISA Kit

Mfr.No.

E1385Ra

Sensitivity

0.026ng/ml

Detection Range

0.05-30ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Nesfatin (also known as NES) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

INV;Involucrin

Molecular Weight

67021

Kit Components

Components	Quantity
Pre-coated ELISA plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E1385Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	12.96	0.42	3.27
Intra-Assay	2	18	6.99	0.39	5.57
Intra-Assay	3	18	3.16	0.24	7.65

Background

Involucrin is a protein component of human skin and in humans is encoded by the IVL gene. In binding the protein loricrin, involucrin contributes to the formation of a cell envelope that protects corneocytes in the skin

Protocol

These standard curves of E1385Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
20ng/ml	2.277	2.346	2.298
	2.416
10ng/ml	1.234	1.303	1.254
	1.372
5ng/ml	0.833	0.796	0.747
	0.758
2.5ng/ml	0.424	0.48	0.431
	0.536
1.25ng/ml	0.264	0.281	0.233
	0.299
0ng/ml	0.046	0.049	0
	0.051

Gene ID	60583
UniProt ID	P48998

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12112619	E1385Ra	48T	$320		Add to Cart
BHE12112619	E1385Ra	96T	$458		Add to Cart

Rat Involucrin - INV ELISA Kit

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