Rat Interleukin 22(IL-22) ELISA Kit

Rat Interleukin 22(IL-22) ELISA Kit

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Mfr.No.

CSB-E14918r

Alternative Names

Interleukin 22(IL-22)

Reactivity

Rat

Detection Range

15.6 pg/mL-1000 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of human TGF-?3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-98
EDTA plasma (n=4)	98	96-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human TGF-?3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	82-95
1:2	Average %	97
	Range %	94-103
1:4	Average %	98
	Range %	90-104
1:8	Average %	87
	Range %	82-91

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.699	2.752	2.726	2.571
500	2.435	2.399	2.417	2.262
250	1.808	1.875	1.842	1.687
125	1.065	1.055	1.060	0.905
62.5	0.678	0.735	0.707	0.552
31.2	0.401	0.422	0.412	0.257
15.6	0.267	0.275	0.271	0.116
0	0.149	0.160	0.155	?

Species

Rattus norvegicus

Sensitivity

3.9 pg/mL

Limit Of Detection

450 nm

UniProt ID	G3V6X6
Antigen	Interleukin 22(IL-22)

Applications

ELISA

Research Area

IL-22

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Increased expression of Th17 cytokines and interleukin-22 correlates with disease activity in pristane-induced arthritis in rats Wang B.et al,PLoS One,Nov

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.