Rat Interleukin 18,IL-18 Elisa Kit

Rat Interleukin 18,IL-18 Elisa Kit

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Mfr.No.

CSB-E04610r

Alternative Names

interleukin 18 (interferon-gamma-inducing factor), IGIF, IL-18, IL-1g, IL1F4, MGC12320, IL-1 gamma, interferon-gamma-inducing factor, interleukin 18, interleukin-1 gamma, interleukin-18

Reactivity

Rat

Detection Range

0.156 ng/mL-10 ng/mL

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse anti-smith antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	85-99
EDTA plasma (n=4)	95	90-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse anti-smith antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	88
	Range %	81-95
1:2	Average %	95
	Range %	90-100
1:4	Average %	93
	Range %	92-101
1:8	Average %	93
	Range %	82-98

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.884	2.752	2.818	2.614
20	2.345	2.276	2.311	2.107
10	1.552	1.495	1.524	1.320
5	0.836	0.801	0.819	0.615
2.5	0.527	0.524	0.526	0.322
1.25	0.321	0.344	0.333	0.129
0.625	0.255	0.259	0.257	0.053
0	0.201	0.206	0.204	?

Species

Rattus norvegicus

Sensitivity

0.039 ng/mL

Limit Of Detection

450 nm

UniProt ID	P97636
Antigen	interleukin 18 (interferon-gamma-inducing factor)

Applications

ELISA

Research Area

IL18

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508198	CSB-E04610r	96T	$496	Add to Cart
BHE10508198	CSB-E04610r	5x96T	$2380.80	Add to Cart
BHE10508198	CSB-E04610r	10x96T	$4712	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.