Rat Interleukin 13 - IL-13 ELISA Kit

Mfr.No.

E0112Ra

Description

This sandwich kit is for the accurate quantitative detection of Rat Interleukin 13 (also known as IL-13) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

IL 13;IL13;IL-13;Interleukin-13;T-cell activation protein P600

Reactivity

Rat

Detection Range

5-1500pg/ml

Sample Type

Serum, Plasma, Cell culture supernatants

Assay Type

Sandwich

Assay Duration

1h 30m

Molecular Weight

14093

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Sensitivity

2.51pg/ml

Background

Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages.

Gene ID	116553
UniProt ID	P42203
Antigen	IL13

Research Area

Immunology

Protocol

These standard curves of E0112Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800pg/ml	2.21	2.087	2.047
	1.965
400pg/ml	1.506	1.501	1.46
	1.497
200pg/ml	0.726	0.706	0.666
	0.687
100pg/ml	0.386	0.398	0.358
	0.41
50pg/ml	0.191	0.162	0.121
	0.133
0pg/ml	0.027	0.041	0
	0.055

Precision

We measured random samples of E0112Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	134	6.33	4.7
Intra-Assay	2	18	321.6	17.33	5.4
Intra-Assay	3	18	416.5	18.8	4.5

Storage	2-8°C

Reference	An experimental model of asthma in rats using ovalbumin and lipopolysaccharide allergens Journal: Heliyon,Volume 5, Issue 11,2019,e02864

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111430	E0112Ra	48T	$320		Add to Cart
BHE12111430	E0112Ra	96T	$458		Add to Cart

Rat Interleukin 13 - IL-13 ELISA Kit

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