Rat Interleukin 13 - IL-13 ELISA Kit

Mfr.No.

E0112Ra

Sensitivity

2.51pg/ml

Detection Range

5-1500pg/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Interleukin 13 (also known as IL-13) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

IL 13;IL13;IL-13;Interleukin-13;T-cell activation protein P600

Molecular Weight

14093

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0112Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	134	6.33	4.7
Intra-Assay	2	18	321.6	17.33	5.4
Intra-Assay	3	18	416.5	18.8	4.5

Background

Cytokine. Inhibits inflammatory cytokine production. Synergizes with IL2 in regulating interferon-gamma synthesis. May be critical in regulating inflammatory and immune responses. Positively regulates IL31RA expression in macrophages.

Protocol

These standard curves of E0112Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
800pg/ml	2.21	2.087	2.047
	1.965
400pg/ml	1.506	1.501	1.46
	1.497
200pg/ml	0.726	0.706	0.666
	0.687
100pg/ml	0.386	0.398	0.358
	0.41
50pg/ml	0.191	0.162	0.121
	0.133
0pg/ml	0.027	0.041	0
	0.055

Gene ID	116553
UniProt ID	P42203

Research Area	Immunology

Storage	2-8°C

Reference	An experimental model of asthma in rats using ovalbumin and lipopolysaccharide allergens Journal: Heliyon,Volume 5, Issue 11,2019,e02864

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111430	E0112Ra	48T	$320		Add to Cart
BHE12111430	E0112Ra	96T	$458		Add to Cart

Rat Interleukin 13 - IL-13 ELISA Kit

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