Rat interferon-inducible protein 10,IP-10 ELISA Kit

Mfr.No.

CSB-E08182r

Alternative Names

chemokine (C-X-C motif) ligand 10, C7, IFI10, INP10, IP-10, SCYB10, crg-2, gIP-10, mob-1, gamma IP10, interferon-inducible cytokine IP-10, protein 10 from interferon (gamma)-induced cell line, small inducible cytokine B10, small inducibl

Reactivity

Rat

Detection Range

781.25 pg/mL-50000 pg/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of mouse OPG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-89
EDTA plasma (n=4)	96	92-99

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse OPG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	87
	Range %	83-90
1:10	Average %	102
	Range %	98-106
1:20	Average %	85
	Range %	81-89
1:40	Average %	97
	Range %	93-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.045	2.102	2.074	1.938
500	1.357	1.308	1.333	1.197
250	0.879	0.915	0.897	0.761
125	0.593	0.578	0.586	0.450
62.5	0.381	0.368	0.375	0.239
31.2	0.275	0.298	0.287	0.151
15.6	0.193	0.187	0.190	0.054
0	0.135	0.137	0.136	?

Species

Rattus norvegicus

Sensitivity

195 pg/mL

Limit Of Detection

450 nm

UniProt ID	P48973
Antigen	chemokine (C-X-C motif) ligand 10

Applications

ELISA

Research Area

CXCL10

Protocol

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays):CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Storage	Store at 2-8°C. Please refer to protocol.

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SKU	Supplier No.	Size	Your price
BHE10508182	CSB-E08182r	96T	$595	Add to Cart
BHE10508182	CSB-E08182r	5x96T	$2856	Add to Cart
BHE10508182	CSB-E08182r	10x96T	$5652.50	Add to Cart

Rat interferon-inducible protein 10,IP-10 ELISA Kit

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