Rat Intercellular Adhesion Molecule 1

Mfr.No.

E0623Ra

Sensitivity

0.021ng/ml

Detection Range

0.05-20ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Description

This sandwich kit is for the accurate quantitative detection of Rat Intercellular adhesion molecule 1 (also known as ICAM-1) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Alternative Names

CD antigen CD54;ICAM 1;ICAM1;ICAM-1;Intercellular adhesion molecule 1

Molecular Weight

60142

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0623Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	1.5	0.09	5.7
Intra-Assay	2	18	6.1	0.18	3
Intra-Assay	3	18	5.4	0.21	4

Background

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity).

Protocol

These standard curves of E0623Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
12ng/ml	2.312	2.359	2.334
	2.406
6ng/ml	1.356	1.307	1.282
	1.258
3ng/ml	0.783	0.747	0.722
	0.71
1.5ng/ml	0.329	0.321	0.297
	0.314
0.75ng/ml	0.129	0.156	0.131
	0.182
0ng/ml	0.017	0.025	0
	0.032

Gene ID	25464
UniProt ID	Q00238

Research Area	Cardiovascular

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111894	E0623Ra	48T	$320		Add to Cart
BHE12111894	E0623Ra	96T	$458		Add to Cart

Rat Intercellular Adhesion Molecule 1 - ICAM-1 ELISA Kit

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