Rat Insulin Receptor Substrate 1

Mfr.No.

E0919Ra

Sensitivity

2.13ng/ml

Detection Range

3-60ng/ml

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

Insulin receptor substrate 1;IRS 1;IRS1;IRS-1;pp185

Molecular Weight

131178

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0919Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	5.1	0.28	5.5
Intra-Assay	2	18	7.3	0.4	5.4
Intra-Assay	3	18	12	0.65	5.4

Background

May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit.

Protocol

These standard curves of E0919Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
40ng/ml	2.287	2.295	2.257
	2.302
20ng/ml	1.942	1.841	1.803
	1.739
10ng/ml	1.212	1.251	1.213
	1.29
5ng/ml	0.625	0.665	0.628
	0.706
2.5ng/ml	0.348	0.348	0.31
	0.348
0ng/ml	0.043	0.038	0
	0.033

Gene ID	25467
UniProt ID	P35570

Research Area	Tags & Cell Markers; Signal Transduction; Neuroscience; Metabolism

Storage	2-8°C

Have you used this product?

Rate it now..!!

SKU	Supplier No.	Size	Your price	Quantity
BHE12112170	E0919Ra	48T	$320		Add to Cart
BHE12112170	E0919Ra	96T	$458		Add to Cart

Rat Insulin Receptor Substrate 1 - IRS-1 ELISA Kit

Contact us to order

Tel

Email

Contact us to order

Tel

Email

Reviews of Rat Insulin Receptor Substrate 1 - IRS-1 ELISA Kit

Rate it now..!!