Rat Insulin-like growth factor binding protein 5,IGFBP-5 ELISA kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E12784r

Sensitivity

0.078 ng/mL

Detection Range

0.312 ng/mL-20 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

insulin-like growth factor binding protein 5, IBP5,

Recovery

The recovery of rat malonyl CoA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	84	80-88

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat malonyl CoA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	98
	Range %	92-103
1:200	Average %	102
	Range %	100-104
1:400	Average %	93
	Range %	88-97
1:800	Average %	89
	Range %	84-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P24594

Applications	ELISA
Research Area	IGFBP5

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Effect of insulin-like growth factor-I during the early postnatal period in intrauterine growtH-restricted rats Ikeda N. et al,Pediatr Int,2015 Dec 4. Effect of insulin-like growth factor-I during the early postnatal period in intrauterine growth-restricted rats. Ikeda N.et al,Pediatr Int.,58(5):353-8

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