Rat Insulin-like growth factor binding protein 5,IGFBP-5 ELISA kit

Rat Insulin-like growth factor binding protein 5,IGFBP-5 ELISA kit

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Mfr.No.

CSB-E12784r

Alternative Names

insulin-like growth factor binding protein 5, IBP5,

Reactivity

Rat

Detection Range

0.312 ng/mL-20 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Type

Sandwich

Assay Duration

1-5h

Recovery

The recovery of rat malonyl CoA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	84	80-88

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat malonyl CoA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:100	Average %	98
	Range %	92-103
1:200	Average %	102
	Range %	100-104
1:400	Average %	93
	Range %	88-97
1:800	Average %	89
	Range %	84-93

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
20	0.102	0.100	0.101
10	0.214	0.206	0.210
5	0.385	0.357	0.371
2.5	0.672	0.619	0.646
1.25	1.121	1.086	1.104
0	1.851	1.729	1.790

Species

Rattus norvegicus

Sensitivity

0.078 ng/mL

Limit Of Detection

450 nm

UniProt ID	P24594
Antigen	insulin-like growth factor binding protein 5

Applications

ELISA

Research Area

IGFBP5

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Effect of insulin-like growth factor-I during the early postnatal period in intrauterine growtH-restricted rats Ikeda N. et al,Pediatr Int,2015 Dec 4. Effect of insulin-like growth factor-I during the early postnatal period in intrauterine growth-restricted rats. Ikeda N.et al,Pediatr Int.,58(5):353-8

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SKU	Supplier No.	Size	Your price
BHE10508171	CSB-E12784r	96T	$695	Add to Cart
BHE10508171	CSB-E12784r	5x96T	$3336	Add to Cart
BHE10508171	CSB-E12784r	10x96T	$6602.50	Add to Cart

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.