Rat Insulin-like growth factor binding protein 2,IGFBP-2 ELISA kit

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E07351r

Alternative Names

insulin-like growth factor binding protein 2, 36kDa, IBP2, IGF-BP53

Reactivity

Rat

Detection Range

0.625 ng/mL-40 ng/mL

Sample Type

Tissue homogenates, Serum, Plasma

Assay Duration

1-5h

Recovery

The recovery of sheep TNF-? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-95
EDTA plasma (n=4)	96	90-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of sheep TNF-? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	95
	Range %	90-99
1:4	Average %	90
	Range %	85-94
1:8	Average %	102
	Range %	98-109

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Sensitivity

0.156 ng/mL

Limit Of Detection

450 nm

UniProt ID	P12843
Antigen	insulin-like growth factor binding protein 2, 36kDa

Applications

ELISA

Research Area

IGFBP2

Precision

Storage	Store at 2-8°C. Please refer to protocol.

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