Rat Insulin-like growth factor binding protein 2,IGFBP-2 ELISA kit

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E07351r

Sensitivity

0.156 ng/mL

Detection Range

0.625 ng/mL-40 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Alternative Names

insulin-like growth factor binding protein 2, 36kDa, IBP2, IGF-BP53

Recovery

The recovery of sheep TNF-? spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-95
EDTA plasma (n=4)	96	90-101

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of sheep TNF-? in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:1	Average %	84
	Range %	80-88
1:2	Average %	95
	Range %	90-99
1:4	Average %	90
	Range %	85-94
1:8	Average %	102
	Range %	98-109

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

UniProt ID	P12843

Applications	ELISA
Research Area	IGFBP2

Storage	Store at 2-8°C. Please refer to protocol.

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