Rat Insulin-like growth factor binding protein 1,IGFBP-1 ELISA Kit

Rat Insulin-like growth factor binding protein 1,IGFBP-1 ELISA Kit

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Mfr.No.

CSB-E07349r

Sensitivity

3.9 pg/mL

Detection Range

15.6 pg/mL-1000 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

insulin-like growth factor binding protein 1, AFBP, IBP1, IGF-BP25, PP12, hIGFBP-1, IGF-binding protein 1, alpha-pregnancy-associated endometrial globulin, amniotic fluid binding protein, binding protein-25, binding protein-26, binding protein-28, gr

Recovery

The recovery of mouse REG3G spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	91-98
EDTA plasma (n=4)	97	92-105

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of mouse REG3G in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	93
	Range %	89-106
1:200	Average %	92
	Range %	83-100
1:400	Average %	95
	Range %	89-99
1:800	Average %	97
	Range %	89-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.190	2.123	2.157	1.996
500	1.793	1.762	1.778	1.617
250	1.295	1.257	1.276	1.115
125	0.779	0.748	0.764	0.603
62.5	0.562	0.554	0.558	0.397
31.25	0.375	0.368	0.372	0.211
15.6	0.263	0.254	0.259	0.098
0	0.157	0.165	0.161

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	P21743

Applications	ELISA
Research Area	IGFBP1

Storage	Store at 2-8°C. Please refer to protocol.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.