Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of INF-alpha-Ab and the recovery rates were calculated by comparing the measured value to the expected amount of INF-alpha-Ab in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
91-104
98
EDTA plasma(n=5)
88-105
97
heparin plasma(n=5)
91-101
97
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of INF-alpha-Ab and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
INF-alpha-Ab ELISA Kit allows for the in vitro quantitative determination of INF-alpha-Ab concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision
Intra-Assay: CV<8% Inter-Assay: CV<10%
Storage
2-8 °C for 6 months
Reviews of Rat INF-alpha-Ab (Interferon-alpha Antibody) ELISA Kit