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Home / ELISA Kit

Rat Indoleamine 2 - 3-dioxygenase - IDO ELISA Kit

SKU : BHE12111990

Catalyzes the first and rate-limiting step in the kynurenine pathway of tryptophan catabolism. Involved in immune regulation.

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Mfr.No.

E0730Ra

Sensitivity

9.25ng/L

Detection Range

20-4500ng/L

Reactivity

Rat

Assay Duration

1h 30m

Assay Type

Sandwich

Alternative Names

IDO 2;IDO2;IDO-2;Indoleamine 2,3-dioxygenase 2;Indoleamine 2,3-dioxygenase-like protein 1;Indoleamine-pyrrole 2,3-dioxygenase-like protein 1

Molecular Weight

45236

Kit Components

Components	Quantity
Pre-coated ELISA Plate	12 * 8 well strips x1
Standard solution	0.5ml x1
Standard diluent	3ml x1
Streptavidin-HRP	6ml x1
Stop solution	6ml x1
Substrate solution A	6ml x1
Substrate solution B	6ml x1
Wash buffer concentrate (25x)	20ml x1
Biotinylated antibody	1ml x1

Precision

We measured random samples of E0730Ra within the same batch/lot to ensure the consistency of the kits' performances.

Intra/Inter-Assay	Sample	n	Mean	Standard Deviation	CV%
Intra-Assay	1	18	514	26.12	5.1
Intra-Assay	2	18	1601.8	70.68	4.4
Intra-Assay	3	18	672.9	27.42	4.1

Background

Catalyzes the first and rate-limiting step in the kynurenine pathway of tryptophan catabolism. Involved in immune regulation.

Protocol

These standard curves of E0730Ra are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Concentration	O.D.	Average	Corrected
2400ng/L	2.139	2.082	2.05
	2.025
1200ng/L	1.425	1.402	1.371
	1.38
600ng/L	0.698	0.718	0.687
	0.738
300ng/L	0.438	0.383	0.352
	0.329
150ng/L	0.128	0.136	0.104
	0.144
0ng/L	0.026	0.032	0
	0.037

Gene ID	681319
UniProt ID	F1LV46

Research Area	Others

Storage	2-8°C

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SKU	Supplier No.	Size	Your price	Quantity
BHE12111990	E0730Ra	48T	$320		Add to Cart
BHE12111990	E0730Ra	96T	$458		Add to Cart

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