Rat Indoleamine 2,3-dioxygenase,IDO ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E13583r

Sensitivity

7.81 pg/mL

Detection Range

31.25 pg/mL-2000 pg/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

indoleamine 2,3-dioxygenase 1, CD107B, IDO, INDO, indole 2,3-dioxygenase, indoleamine-pyrrole 2,3 dioxygenase

Recovery

The recovery of rat DA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-95
EDTA plasma (n=4)	96	94-98

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat DA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
?	Sample	Serum(n=4)
1:5	Average %	86
	Range %	84-96
1:10	Average %	95
	Range %	91-100
1:20	Average %	84
	Range %	81-88
1:40	Average %	92
	Range %	88-96

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

UniProt ID	Q9ERD9

Applications	ELISA
Research Area	IDO1

Storage	Store at 2-8°C. Please refer to protocol.

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