Rat Immunoglobulin G,IgG ELISA Kit

Rat Immunoglobulin G,IgG ELISA Kit

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Mfr.No.

CSB-E07981r

Alternative Names

Immunoglobulin G,IgG

Reactivity

Rat

Detection Range

31.25 ng/mL-8000 ng/mL

Sample Type

Serum, Plasma

Assay Type

Competitive

Assay Duration

1-5h

Recovery

The recovery of rat FGF21 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	80-90
EDTA plasma (n=4)	96	91-103

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat FGF21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-102
1:2	Average %	91
	Range %	86-97
1:4	Average %	106
	Range %	102-114
1:8	Average %	95
	Range %	90-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1500	2.646	2.772	2.709	2.590
750	1.973	1.932	1.953	1.834
375	1.217	1.302	1.260	1.141
187.5	0.789	0.793	0.791	0.672
94	0.463	0.449	0.456	0.337
47	0.302	0.281	0.292	0.173
23.5	0.195	0.205	0.200	0.081
0	0.121	0.117	0.119

Species

Rattus norvegicus

Sensitivity

31.25 ng/mL

Limit Of Detection

450 nm

Antigen	Immunoglobulin G,IgG

Applications

ELISA

Research Area

IgG

Protocol

Precision

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Influence of Different Polypeptides Fractions Derived from Sus Scrofa Immune Organs on the Rats Immunological Reactivity L.V. Fedulova.et al,Journal of Pharmacy and Nutrition Sciences,Apr 25 Modulation of Immune Function in Rats Using Oligosaccharides Extracted from Palm Kernel Cake Mohammd Faseleh Jahromi.et al,BioMed Research International,/ The cardioprotective and antioxidant effects of dimethyl-5-(bioguanide-1-il)isophthalate under the conditions of cardiovascular pathology and experimental rheumatoid arthritis in rats T. N. Popova.et al,Russian Journal of Bioorganic Chemistry,Aug 2

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.