Rat Immunoglobulin E,IgE ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

Mfr.No.

CSB-E07984r

Sensitivity

0.78 ng/mL

Detection Range

3.12 ng/mL-200 ng/mL

Reactivity

Rat

Assay Duration

1-5h

Assay Type

Sandwich

Alternative Names

Immunoglobulin E,IgE

Recovery

The recovery of human MAGE-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	104	92-107
EDTA plasma (n=4)	96	88-100

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human MAGE-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	89-101
1:2	Average %	95
	Range %	87-99
1:4	Average %	88
	Range %	82-93
1:8	Average %	97
	Range %	91-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

Species

Rattus norvegicus

Limit Of Detection

450 nm

Precision

Protocol

Applications	ELISA
Research Area	IgE

Storage	Store at 2-8°C. Please refer to protocol.

Reference	Asthma-like airway inflammation and responses in a rat model of atopic dermatitis induced by neonatal capsaicin treatment Han RT.et al,J Asthma Allergy. ,May 18 Modulation of Immune Function in Rats Using Oligosaccharides Extracted from Palm Kernel Cake Mohammd Faseleh Jahromi.et al,BioMed Research International,/ Effects of the combined extracts of Herba Epimedii and Fructus Ligustrilucidi on airway remodeling in the asthmatic rats with the treatment of budesonide Tang X.et al,BMC Complement Altern Med.,Aug 1

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