Rat IKBalpha(Inhibitory Subunit of NF-kappaBalpha) ELISA Kit

Mfr.No.

ER1084

Alternative Names

IKBalpha ELISA Kit, MAD-3 ELISA Kit, NFKBIA ELISA Kit, alpha ELISA Kit, I-kappa-B-alpha ELISA Kit, IkappaBalphaNF-kappa-B inhibitor alpha ELISA Kit, IKBAikB-alpha ELISA Kit, IkB-alpha ELISA Kit, MAD3 ELISA Kit, MAD-3 ELISA Kit, Major histocompatibility complex enhancer-binding protein MAD3 ELISA Kit, NFKBI ELISA Kit, NFKBIA ELISA Kit, nuclear factor of kappa light chain gene enhancer in B-cells ELISA Kit, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor ELISA Kit

Reactivity

Rat

Detection Method

Double Antibody

Detection Range

0.313-20ng/ml

Sample Type

Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate

Assay Type

Sandwich

Recovery

Matrices listed below were spiked with certain level of IKBalpha and the recovery rates were calculated by comparing the measured value to the expected amount of IKBalpha in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	88-98	94
EDTA plasma(n=5)	88-105	96
heparin plasma(n=5)	85-104	95

Linearity

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IKBalpha and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	92-104%	85-95%	87-92%
EDTA plasma(n=5)	85-101%	83-99%	93-100%
heparin plasma(n=5)	83-100%	83-89%	81-99%

Sensitivity

< 0.188ng/ml

Components

Microplate,Lyophilized Standard,buffer,SABC,Label antibody,SABC,TMB,stop solution

Background

IKBα/MAD-3/NFKBIA/alpha/I-kappa-B-alpha/IkappaBalphaNF-kappa-B inhibitor alpha/IKBAikB-alpha/IkB-alpha/MAD3/MAD-3/Major histocompatibility complex enhancer-binding protein MAD3/NFKBI/NFKBIA/nuclear factor of kappa light chain gene enhancer in B-cells/nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor

Notes

For Research Use Only

UniProt ID	Q63746
Antigen	IKBα

Applications	ELISA
Applications Notes	IKBalpha ELISA Kit allows for the in vitro quantitative determination of IKBalpha concentrations in serum, plasma, tissue homogenates and other biological fluids.
Precision	Intra-Assay: CV<8% Inter-Assay: CV<10%

Storage	2-8 °C for 6 months

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SKU	Supplier No.	Size	Your price	Quantity
BHE10804447	ER1084	96T	$400		Add to Cart

Rat IKBalpha (Inhibitory Subunit of NF-kappaBalpha) ELISA Kit

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