Other liquid samples, Serum, Cell culture supernatants, Plasma, Cell lysate
Assay Type
Sandwich
Recovery
Matrices listed below were spiked with certain level of IgG1 and the recovery rates were calculated by comparing the measured value to the expected amount of IgG1 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
87-104
98
EDTA plasma(n=5)
87-104
97
heparin plasma(n=5)
85-104
95
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of IgG1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-101%
98-105%
95-102%
EDTA plasma(n=5)
85-101%
88-96%
86-98%
heparin plasma(n=5)
93-98%
85-97%
85-98%
Sensitivity
< 0.938ng/ml
Notes
For Research Use Only
Applications
ELISA
Applications Notes
IgG1 ELISA Kit allows for the in vitro quantitative determination of IgG1 concentrations in serum, plasma, tissue homogenates and other biological fluids.